The role of Scientist I/II (Upstream) is to support TNACs R&D function by following SOPs as directed by senior staff in areas such as molecular biology cell culture and protein purification.
The role will be mainly labbased.
Key roles and responsibilities include but are not limited to: Working proficiently and independently to existing SOPs in the following fields: Molecular Biology.
Construction of expression vectors using standard techniques such as: RNA and DNA extraction; preparative and analytical PCR; reversetranscription PCR; restriction digestion; agarose electrophoresis; Gibson assembly; preparation of transfectiongrade plasmids at various scales; Sanger and Nanopore sequencing.
Bacterial cell culture.
Preparation of growth media; transformation of bacteria; expression testing and optimisation of culture conditions; native and recombinant protein expression at various scales; harvesting and QC testing of raw materials.
Mammalian cell culture.
Maintenance of cell lines; performing small and largescale transfections; harvesting and QC testing of raw materials.
Protein purification and analysis.
Preparation of suitable buffers; smallscale FPLC purification of target proteins using spin columns; SDSPAGE; Western blotting; and ELISA.
Daytoday reporting of results to line manager(s) as required.
Helping with ordering and maintaining stock levels of highturnover items and reagents.
Meticulous recordkeeping.
Weekly supervisor meetings.
Attendance and presentation at weekly lab meetings.
Developing an ability to understand and convey relevant published research.
Additional duties as required by senior R&D staff.
The Scientist I/II (Upstream) will also be able to demonstrate the following traits in line with LGCs Core Values: Passion: Pride in our achievements engenders passion for our work and translates into our shared ambition to make the world a safer place.
Curiosity : We constantly look forward to whats next: our curiosity for todays discoveries makes tomorrows innovations possible.
Integrity : A commitment to high ethical standards has been at the heart of LGC since we first started testing the integrity of products in 1842.
Brilliance: We strive to be the very best in everything we say and do.
We exceed customers expectations through innovation and pioneering science.
Respect: We respect gender age nationality religion and individuality our diversity is our strength.
Qualifications : A life sciencesbased degree Remote Work : No Employment Type : Fulltime